D
D. ZF21, 5-caccgcagtgtgacgccaagtttgacgaatcaaacttggcgtcacactgc-3; FAK1, 5-caccgcatcttccagttacaaattccgaagaatttgtaactggaagatgc-3, and FAK2, 5-caccgccaacccatctgatggaagacgaatcttccatcagatgggttggc-3. shRNA expressing lentiviral vectors were generated and used according to the manufacturer’s instructions (Invitrogen). For rescue experiments of ZF21 knockdown, an expression construct encoding a mutant ZF21 mRNA refractory to shRNA was generated by site-directed mutagenesis, and the mutant protein was expressed with a Myc tag at…