(D) Distribution and the common abscission period of HeLa cells transiently transfected with siRNA-Ric-8A or siRNA-control as well as the photo-activable GFP plasmid

(D) Distribution and the common abscission period of HeLa cells transiently transfected with siRNA-Ric-8A or siRNA-control as well as the photo-activable GFP plasmid. 22 pieces z-stack, 1 picture every 3 min). 3D reconstruction was performed using Imaris and films (2 fps) had been synchronized using Adobe Premiere. Size bar is certainly 5 m.(MOV) pone.0086680.s003.mov (8.1M)…