After buffer exchange to remove unreacted DTT, the partially reduced antibody was reacted with a 10 fold molar excess of maleimide activated MC-VC-PAB-MMAE to generate the crude 10D7-MMAE product
After buffer exchange to remove unreacted DTT, the partially reduced antibody was reacted with a 10 fold molar excess of maleimide activated MC-VC-PAB-MMAE to generate the crude 10D7-MMAE product. 807 residues including a 637 residue amino-terminal extracellular domain name (ECD), a 20 residue transmembrane domain name, and a 150 residue carboxyl-terminal intracellular domain name 25,…