In the holistic and systemic context, proteomics have a convergence with traditional Chinese medicine (TCM) syndrome, which could overcome the one-sidedness and singleness of TCM and avoid the complication and tedious processes. as samples the serum of healthy persons and patients with liver depressive disorder diseases, and separating proteins by two-dimensional gel electrophoresis. After the comparison between the normal groups and liver depressive disorder groups, the differential protein group was found and 12 differential protein spots were recognized. The further analysis exhibited these differential proteins were mainly related to immunization, neuroendocrine and nutrient metabolism , consistent with the existing research findings. Certain achievements in the filed have been UAMC 00039 dihydrochloride acquired (Table 3). Table 3 Application of proteomics in the studies of TCM syndrome decoction, the expression of those proteins was adjusted to normal levels. The adjustive action of dry of Chinese traditional medicine on proteomics of small intestinal epithelia is one of the important mechanism of invigorating spleen of dry em Radix Ginseng /em .28Tianma Gouteng Decoction?Two-dimensional gel electrophoresis, MALDI-TOF-MS and database analysisAbout 20 protein spots were expressed at different levels between normal and disease groups. Among these spots, five were up-regulated twofold and 10 were down-regulated at ISGF3G least twofold in disease group. After being treated Tianmai Gouteng Decoction, the expression of those proteins was adjusted to the normal levels.29Total flavones of hippophae rhamnoides L. and choerospondias axillaries fructusProteins from myocardial ischaemia Surface enhanced laser desorption/ionization mass spectrometry, protein chip (IMAC3, SAX2 and NP20)Six differentially expressed proteins were recognized in total flavones of hippophae rhamnoides L. (TFH) treated myocardium, in which five down-regulated and one up-regulated. Seven differentially expressed proteins were recognized in total flavones of choreospondias axillaries fructus (TFC) treated myocardium, among these proteins, five down-regulated and two up-regulated. The myocardial protection of TFH and TFC may be mediated by UAMC 00039 dihydrochloride the differential expression of these proteins which could be the key protein candidates for different kinds and amounts.30Qi-Boosting Toxin-Resolving GranuleProteins from HNEI cells Two-dimensional gel electrophoresis, silver staining and PDQuest 2-de microsoftThe mean quantity of protein spots was 1120 89 for treated HNEI cells with Qi-Boosting Toxin-Resolving Granule and that was 1281 102 for untreated cells, the differentially expressed protein spots were 673, among which 218 were up-regulated whereas 455 down-regulated in treated HNEI cells.31 Open in a separate window *Guizhi Decoction is one of the most famous prescription, first recorded in the Treatise on Cold-induced Febride Diseases. It was made up of ramulus cinnamomi, paeonia lactiflora, radix glycyrrhiza, rhizome zingiberis recens and ziziphus jujube Mill. ?Tianma Gouteng Decoction contains gastrodia elata Blume, gardenia jasminoides Ellis, scutellaria baicalensis Georgi, eucommia ulmoides Oliver, leonurus japonicus Houtt, Chinese taxillus Twing, caulis polygoni Multiflori, cyathula officinalis Kuan and ramulus uncariae cumuncis. Problems and potential customers Being constrained by research tools, levels of acknowledgement, analytical methods and other factors, the endeavour to expose proteomics into the studies of TCM symptoms is still confronted with many troubles. Currently, experts mainly apply proteomics technology to detect and identify differential proteins. However, the investigation of protein function is still not gone much enough. Although abundant proteomics responsible for TCM syndrome have been discovered, UAMC 00039 dihydrochloride the problem what the role these proteomics play in the generation and development has not yet been resolved. Despite of the rapid advancements acquired by proteomics in the past years, there are also some disadvantages: low reproducibility, difficult separation of proteins of big or small isoelectric point. Moreover, some hydrophobic proteins, insoluble membrane proteins and large molecular weight, low-abundance proteins may be ignored in the process of examination. The notion of TCM syndrome possesses the obvious macroscopic. The scope of TCM symptoms commonly covers multiple organs, organization or functional.