Tianjin, China) was used to detect IgG antibody, and the experimental process followed the instructions

Tianjin, China) was used to detect IgG antibody, and the experimental process followed the instructions. and CPA in non-agranulocytic patients. Methods Fifty-eight cases of pulmonary aspergillosis (37 IPA and 21 CPA cases), 15 cases of community-acquired bacterial pneumonia and 50 cases in the healthy control group were collected. The serum (1,3)–D-glucan test (G test) was performed with a chromogenic method, and the galactomannan test (GM test) and IgG and IgM antibody detection were performed by commercial enzyme-linked immunosorbent assay (ELISA) in all patients. The sensitivity and specificity, cut-off value and area under the curve (AUC) of IgG and IgM antibodies were further obtained by receiver operating characteristic (ROC) curves. Results The positive rate of the G test, IgG antibody detection and the GM test also showed notable differences among the IPA, CPA, community-acquired bacterial pneumonia and healthy groups (IgG antibody detection had a higher specificity in the IPA group than in the CPA group (0.952). The detection of IgG antibody can preferably distinguish IPA from community-acquired bacterial pneumonia and healthy controls (sensitivity?=?0.923, specificity?=?0.459, cut-off value?=?134.46, AUC?=?0.727). It can also distinguish CPA from community-acquired bacterial pneumonia and healthy controls (sensitivity?=?0.952, specificity?=?0.692, cut-off value?=?75.46, AUC?=?0.873). Conclusions Serum IgG antibody detection may have certain clinical value in the diagnosis of IPA and CPA in non-agranulocytic patients. IgG, Diagnosis Background Pulmonary aspergillosis is usually a type of lung disease caused by contamination or the inhalation of antigen. Pulmonary aspergillosis is usually uncommon in non-agranulocytic patients, and only a small amount of data are available. Nevertheless, in recent years, the incidence of pulmonary aspergillosis in non-granulocytic patients has increased with ageing; the increase in chronic diseases; the use of broad-spectrum antibiotics, hormones, and immunosuppressive drugs; and invasive operations [1, 2]. Moreover, the Rabbit polyclonal to ATL1 clinical manifestations of these patients lack specificity, and the diagnosis is usually hard, which leads to treatment delay and affects the prognosis. According to the clinical characteristics, pulmonary aspergillosis can be divided into allergic bronchopulmonary aspergillosis (ABPA), chronic pulmonary aspergillosis (CPA), invasive pulmonary aspergillosis (IPA), and subacute invasive aspergillosis (SAIA) [3]. Among them, CPA usually occurs in immunocompetent individuals with underlying respiratory disorders, and the prevalence of CPA worldwide is usually approximately 3 million [4]. GSK2795039 Unfortunately, respiratory physicians may not detect CPA until the disease progresses to an advanced stage owing to the lack of GSK2795039 specific clinical manifestations. More seriously, without timely diagnosis and long-term antifungal treatment, the 5-12 months mortality rate of patients with CPA reaches 80% [5]. Furthermore, invasive pulmonary aspergillosis (IPA) has become a common type of severe pneumonia with the highest mortality, and one of the important reasons the is usually difficulty in diagnosis [6]. In addition, patients with agranulocytosis are predominant among those with IPA, and relevant international guidelines for diagnosis and treatment also focus on them [7]. The diagnosis of pulmonary aspergillosis depends on histopathology and microbiological culture, but you will find risks in obtaining tissue specimens. Traditional microbiological culture has a low positive rate, takes a long time, and has the possibility of contamination and colonization. However, serological diagnosis as a non-invasive diagnostic method is usually conducive to the early diagnosis of pulmonary aspergillosis but avoids over-diagnosis. However, GSK2795039 this method has a false-positive reaction during the detection process, which reduces the sensitivity. The method has the advantages of high efficiency and time savings, high specificity, and high sensitivity and is suitable for the detection of a large number of samples. IgM antibody has a short half-life and disappears quickly; therefore, it can be detected in blood as an indication of recent contamination. IgG antibodies are characterized by late production, long maintenance time, slow disappearance and high concentration. Therefore, its detection in blood can be used as an indication of long-term contamination. Among the serological diagnoses, it is well known that serum IgG and IgM antibody detection is mainly used in the clinical diagnosis of CPA [8]. Related research exhibited that (cell wall. However, these two tests have low positive rate and poor sensitivity in non-agranulocytic patients. In this study, we explored the value of the G test, GM test, and serum IgG and IgM antibody detection for the diagnosis of IPA and CPA in non-agranulocytic patients. Methods Patients and data collection Fifty-eight pulmonary aspergillosis cases in non-agranulocytic patients admitted to Tianjin Chest Hospital from July 2017 to July 2018 were enrolled. The diagnostic criteria referred to the consensus of experts in the diagnosis and treatment of pulmonary mycosis and the criteria of the European Organization for Research and Treatment of Malignancy (EORTC) [11, 12]. The exclusion criteria were as follows: (1) agranulocytic patients, (2) patients with other lung diseases, (3) patients with.