Downward circulation may not affect MACS with CD34 mAb because antibodies generally have a 10,000-fold higher ligand-binding affinity than TCRs (Matsui em et al
Downward circulation may not affect MACS with CD34 mAb because antibodies generally have a 10,000-fold higher ligand-binding affinity than TCRs (Matsui em et al. /em , 1991). In agreement with these findings, Streptamer, the only detachable reagent, exposed significant T cell growth in the 1st week after MACS. Sorting TCR and tCD34 gene-engineered T cells…