Beads were washed thrice in PBS containing protease inhibitors, eluted in 2x SDS test buffer (125 mM Tris-HCl, 20% glycerol, 4% SDS, and 0
Beads were washed thrice in PBS containing protease inhibitors, eluted in 2x SDS test buffer (125 mM Tris-HCl, 20% glycerol, 4% SDS, and 0.005% bromphenol blue), heated to 95C, and analyzed by immunoblotting. For immunoprecipitation analysis, the cell lysates from transfected HEK-293 cells were incubated using the indicated antibodies and proteins A- and proteins G…