The images shown in (A,B) are representative of three independent experiments
The images shown in (A,B) are representative of three independent experiments. quantification of nascent RNA molecules excludes that the downmodulation arises in the transcription initiation step, rather pointing towards a post-transcriptional mechanism. Indeed, a significantly higher fraction of unspliced mRNA is detected in ubiquitin overexpressing cells, compared to empty vector transfected cells. Our findings suggest…